Evaluation of catechin, lupeol, and betulinic acid as markers for the chromatographic quality Control of Albizia coriaria raw materials; an experimental study

Albizia coriaria stembark is among the most common raw materials used to manufacture herbal products in Uganda. While plant material sourced from wild, there are neither monographs nor chemical analytical methods for its standardisation. In addition, good cultivation, harvesting, and manufacturing practices not mandatory. This leads inconsistent quality of products. work developed TLC HPLC evaluated suitability lupeol, catechin, betulinic acid markers standardization A. coriaria. Samples were extracted by decoction, maceration soxhlet reflux; water, ethanol ethyl acetate. Catechin, lupeol found all batches different agroecological zones, at concentrations higher than 6mg/g. Ethyl acetate fractions gave highest marker quantities, while decoction lowest. The optimised fingerprint conditions solvent system: 4, 2, 1 parts n-hexane, acetate, dichloromethane, methanol, respectively; stationary phase; glass plates, 10 cm × 20 cm, coated with fluorescent 60Å silica gel matrices vanillin / sulfuric spray reagent. resulting fingerprints comprised twelve principal peaks. optimized quantitation method were: C18 column (250 4.6 mm x 5 µm), system; acetonitrile (99.99%) 1% trifluoroacetic (0.01%); flow rate, 1mL/min; temperature, 25°C; UV/visible detector 205 nm. had correlation coefficient >0.99, quantitative accurate (>97% recovery) reproducible (RSD < 0.38%). Lupeol suitable control materials. decoctions better controlled catechin. Key words: Markers, fingerprints, coriaria, acid, control, standardisation